{"product_id":"proteintech-66668-1-ig","title":"Proteintech, 66668-1-Ig, PLCB3 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PLCB3 (66668-1-Ig) by Proteintech is a Monoclonal antibody targeting PLCB3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n66668-1-Ig targets PLCB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, Jurkat cells,  HSC-T6 cells,  NIH\/3T3 cells,  HeLa cells,  Sp2\/0 cells,  human peripheral blood platelets,  HEK-293 cells,  K-562,  LNCaP cells,  K-562 cells\nPositive IHC detected in: human breast cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq\/11 and\/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag15845 Product name: Recombinant human PLCB3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 885-1234 aa of BC142681 Sequence: AQAGQETCQDTQSQQLGSQPSSNPTPSPLDASPRRPPGPTTSPASTSLSSPGQRDDLIASILSEVAPTPLDELRGHKALVKLRSRQERDLRELRKKHQRKAVTLTRRLLDGLAQAQAEGRCRLRPGALGGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREAQVDAEAQRRLEHLRQALQRLREVVLDANTTQFKRLKEMNEREKKELQKILDRKRHNSISEAKMRDKHKKEAELTEINRRHITESVNSIRRLEEAQKQRHDRLVAGQQQVLQQLAEEEPKLLAQLAQECQEQRARLPQEIRRSLLGEMPEGLGDGPLVACASNGHAPGSSGHLSGADSESQEENTQL Predict reactive species\nFull Name: phospholipase C, beta 3 (phosphatidylinositol-specific)\nCalculated Molecular Weight: 1234 aa, 139 kDa\nObserved Molecular Weight: 150 kDa\nGenBank Accession Number: BC142681\nGene Symbol: PLCB3\nGene ID (NCBI): 5331\nRRID: AB_2882022\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: Q01970\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888012513449,"sku":"66668-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-66668-1-ig","provider":"Iright","version":"1.0","type":"link"}