{"product_id":"proteintech-66732-1-ig","title":"Proteintech, 66732-1-Ig, ARNT\/HIF-1 beta Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe ARNT\/HIF-1 beta (66732-1-Ig) by Proteintech is a Monoclonal antibody targeting ARNT\/HIF-1 beta in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n66732-1-Ig targets ARNT\/HIF-1 beta in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Caco-2 cells, HeLa cells,  HepG2 cells,  HT-29 cells,  HSC-T6 cells,  NIH\/3T3 cells\nPositive IF\/ICC detected in: A549 cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:1000-1:4000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1\/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1\/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag5507 Product name: Recombinant human ARNT,HIF1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-348 aa of BC060838 Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS Predict reactive species\nFull Name: aryl hydrocarbon receptor nuclear translocator\nCalculated Molecular Weight: 416 aa, 46 kDa, 87 kDa\nObserved Molecular Weight: 87 kDa\nGenBank Accession Number: BC060838\nGene Symbol: ARNT\/HIF-1 beta\nGene ID (NCBI): 405\nRRID: AB_2882082\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P27540\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888100102313,"sku":"66732-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-66732-1-ig","provider":"Iright","version":"1.0","type":"link"}