{"product_id":"proteintech-66757-1-ig","title":"Proteintech, 66757-1-Ig, AURKA Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe AURKA (66757-1-Ig) by Proteintech is a Monoclonal antibody targeting AURKA in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n66757-1-Ig targets AURKA in WB, IHC, IF\/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human testis tissue, HeLa cells,  NCCIT cells,  rat testis tissue,  mouse testis tissue\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser\/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and\/or telophase by being involved in microtubule formation and\/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Mouse \/ IgG3\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag20096 Product name: Recombinant human AURKA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 108-337 aa of BC002499 Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET Predict reactive species\nFull Name: aurora kinase A\nCalculated Molecular Weight: 46 kDa\nObserved Molecular Weight: 46-52 kDa\nGenBank Accession Number: BC002499\nGene Symbol: AURKA\nGene ID (NCBI): 6790\nRRID: AB_2882103\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O14965\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887986004137,"sku":"66757-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-66757-1-ig","provider":"Iright","version":"1.0","type":"link"}