{"product_id":"proteintech-67699-1-ig","title":"Proteintech, 67699-1-Ig, PPT1 Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PPT1 (67699-1-Ig) by Proteintech is a Monoclonal antibody targeting PPT1 in WB, IF-P, ELISA applications with reactivity to human, pig, rabbit samples\n67699-1-Ig targets PPT1 in WB, IF-P, ELISA applications and shows reactivity with human, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, pig heart tissue,  pig brain tissue,  LNCap cells,  HeLa cells,  Jurkat cells,  K-562 cells,  pig cerebellum tissue,  rabbit brain tissue,  rabbit cerebellum tissue\nPositive IF-P detected in: mouse testis tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, pig, rabbit\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag30550 Product name: Recombinant human PPT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-306 aa of BC008426 Sequence: MASPGCLWLLAVALLPWTCASRALQHLDPPAPLPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIYVLSLEIGKTLMEDVENSFFLNVNSQVTTVCQALAKDPKLQQGYNAMGFSQGGQFLRAVAQRCPSPPMINLISVGGQHQGVFGLPRCPGESSHICDFIRKTLNAGAYSKVVQERLVQAEYWHDPIKEDVYRNHSIFLADINQERGINESYKKNLMALKKFVMVKFLNDSIVDPVDSEWFGFYRSGQAKETIPLQETSLYTQDRLGLKEMDNAGQLVFLATEGDHLQLSEEWFYAHIIPFLG Predict reactive species\nFull Name: palmitoyl-protein thioesterase 1\nCalculated Molecular Weight: 34 kDa\nObserved Molecular Weight: 32-34 kda\nGenBank Accession Number: BC008426\nGene Symbol: PPT1\nGene ID (NCBI): 5538\nRRID: AB_2882891\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P50897\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888313487529,"sku":"67699-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-67699-1-ig","provider":"Iright","version":"1.0","type":"link"}