{"product_id":"proteintech-67751-1-ig","title":"Proteintech, 67751-1-Ig, PRKAR2A Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe PRKAR2A (67751-1-Ig) by Proteintech is a Monoclonal antibody targeting PRKAR2A in WB, IHC, IF\/ICC, ELISA applications with reactivity to Human, rat, mouse samples\n67751-1-Ig targets PRKAR2A in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U2OS cells, NIH\/3T3 cells,  HSC-T6 cells,  Jurkat cells,  HepG2 cells,  HeLa cells,  LNCaP cells\nPositive IHC detected in: human colon cancer tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:400-1:1600\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit  of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2.  The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: Human, rat, mouse\nCited Reactivity: human\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag29893 Product name: Recombinant human PRKAR2A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 74-382 aa of BC002763 Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ Predict reactive species\nFull Name: protein kinase, cAMP-dependent, regulatory, type II, alpha\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 50 kDa\nGenBank Accession Number: BC002763\nGene Symbol: PRKAR2A\nGene ID (NCBI): 5576\nRRID: AB_2918520\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P13861\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888071954601,"sku":"67751-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-67751-1-ig","provider":"Iright","version":"1.0","type":"link"}