{"product_id":"proteintech-67826-1-ig","title":"Proteintech, 67826-1-Ig, Phospho-P53 (Ser15) Monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-P53 (Ser15) (67826-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-P53 (Ser15) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n67826-1-Ig targets Phospho-P53 (Ser15) in WB, IHC, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated HT-29 cells, HT-29 cells,  HEK-293 cells,  HEK-293t cells,  Etoposide treated  HT-29 cells,  Calyculin A treated HEK-293 cells,  UV treated HEK-293T cells\nPositive IF\/ICC detected in: etoposide treated HT-29 cells\nPositive FC (Intra) detected in: UV treated HT-29 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. \nTP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Mouse \/ IgG1\nClass: Monoclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tumor protein p53\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC003596\nGene Symbol: P53\nGene ID (NCBI): 7157\nRRID: AB_2918588\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein G purification\nUNIPROT ID: P04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887988592809,"sku":"67826-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-67826-1-ig","provider":"Iright","version":"1.0","type":"link"}