{"product_id":"proteintech-68719-1-ig","title":"Proteintech, 68719-1-Ig, ALPL Monoclonal antibody","description":"Size: 20ul \/ 150ul\nThe ALPL (68719-1-Ig) by Proteintech is a Monoclonal antibody targeting ALPL in WB, IHC, ELISA applications with reactivity to human, pig, rabbit samples\n68719-1-Ig targets ALPL in WB, IHC, ELISA applications and shows reactivity with human, pig, rabbit samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: pig kidney tissue, rabbit kidney tissue\nPositive IHC detected in: rat adrenal gland tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThere are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver\/bone\/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, pig, rabbit\nHost \/ Isotype: Mouse \/ IgG2a\nClass: Monoclonal\nType: Antibody\nImmunogen: CatNo: Ag33678 Product name: Recombinant human ALPL protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 23-330 aa of BC021289 Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL Predict reactive species\nFull Name: alkaline phosphatase, liver\/bone\/kidney\nCalculated Molecular Weight: 57 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC021289\nGene Symbol: ALPL\nGene ID (NCBI): 249\nRRID: AB_3670412\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P05186\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888086864041,"sku":"68719-1-Ig","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-68719-1-ig","provider":"Iright","version":"1.0","type":"link"}