{"product_id":"proteintech-80077-2-rr","title":"Proteintech, 80077-2-RR, Phospho-P53 (Ser392) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-P53 (Ser392) (80077-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-P53 (Ser392) in WB, FC (Intra), ELISA applications with reactivity to human samples\n80077-2-RR targets Phospho-P53 (Ser392) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: λ phosphatase treated HEK-293 cells\nPositive FC (Intra) detected in: λ phosphatase treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nP53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and\/or enhanced degradation. (PMID: 19935675, PMID: 24379683)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: tumor protein p53\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC003596\nGene Symbol: P53\nGene ID (NCBI): 7157\nRRID: AB_3670464\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888803500201,"sku":"80077-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80077-2-rr","provider":"Iright","version":"1.0","type":"link"}