{"product_id":"proteintech-80084-1-rr","title":"Proteintech, 80084-1-RR, Phospho-Beta Catenin (Ser675) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Beta Catenin (Ser675) (80084-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Beta Catenin (Ser675) in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n80084-1-RR targets Phospho-Beta Catenin (Ser675) in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293,  λ phosphatase treated NIH\/3T3 cells\nPositive IF\/ICC detected in: λ phosphatase treated MCF-7 cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nβ-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt\/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: catenin (cadherin-associated protein), beta 1, 88kDa\nCalculated Molecular Weight: 781 aa, 86 kDa\nObserved Molecular Weight: 90-100 kDa\nGenBank Accession Number: BC058926\nGene Symbol: Beta Catenin\nGene ID (NCBI): 1499\nENSEMBL Gene ID: ENSG00000168036\nRRID: AB_2918863\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P35222\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888363753641,"sku":"80084-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80084-1-rr","provider":"Iright","version":"1.0","type":"link"}