{"product_id":"proteintech-80209-6-rr","title":"Proteintech, 80209-6-RR, Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-AMPK Alpha (Thr172) (80209-6-RR) by Proteintech is a Recombinant antibody targeting Phospho-AMPK Alpha (Thr172) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples\n80209-6-RR targets Phospho-AMPK Alpha (Thr172) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: H2O2 treated C2C12 cells, λ phosphatase treated HEK-293 cells\nPositive FC (Intra) detected in: λ phosphatase treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAMPK is a serine\/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP\/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP\/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an \"energy gauge\". (PMID: 27034026, PMID: 21980456, PMID: 27600021)This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: protein kinase, AMP-activated, alpha 1 catalytic subunit\nObserved Molecular Weight: 64 kDa\nGenBank Accession Number: BC048980\nGene Symbol: AMPK Alpha 1\nGene ID (NCBI): 5562\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13131\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888683569321,"sku":"80209-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80209-6-rr","provider":"Iright","version":"1.0","type":"link"}