{"product_id":"proteintech-80575-3-rr","title":"Proteintech, 80575-3-RR, Phospho-S6 Ribosomal protein (Ser240\/244) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-S6 Ribosomal protein (Ser240\/244) (80575-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-S6 Ribosomal protein (Ser240\/244) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n80575-3-RR targets Phospho-S6 Ribosomal protein (Ser240\/244) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 20% fetal bovine serum after serum free NIH\/3T3 cells, 10% fetal bovine serum after serum free HEK-293 cells,  20% fetal bovine serum after serum free PC-12 cells\nPositive IF\/ICC detected in: HepG2 cells, Rapamycin treated HepG2 cells\nPositive FC (Intra) detected in: 10% fetal bovine serum treated HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRibosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and\/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235\/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235\/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: ribosomal protein S6\nCalculated Molecular Weight: 29 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC000524\nGene Symbol: RPS6\nGene ID (NCBI): 6194\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P62753\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888804352169,"sku":"80575-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80575-3-rr","provider":"Iright","version":"1.0","type":"link"}