{"product_id":"proteintech-80629-2-rr","title":"Proteintech, 80629-2-RR, Phospho-MEK1 (Thr286) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-MEK1 (Thr286) (80629-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-MEK1 (Thr286) in WB, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n80629-2-RR targets Phospho-MEK1 (Thr286) in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: nocodazole treated NIH\/3T3 cells\nPositive IF\/ICC detected in: nocodazole treated NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK\/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217\/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1\/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase kinase 1\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 40-45 kDa\nGenBank Accession Number: BC139729\nGene Symbol: MEK1\nGene ID (NCBI): 5604\nENSEMBL Gene ID: ENSG00000169032\nRRID: AB_3670482\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q02750\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888803008681,"sku":"80629-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80629-2-rr","provider":"Iright","version":"1.0","type":"link"}