{"product_id":"proteintech-80694-2-rr","title":"Proteintech, 80694-2-RR, Phospho-YAP1 (Ser127) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-YAP1 (Ser127) (80694-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-YAP1 (Ser127) in WB, FC (Intra), ELISA applications with reactivity to human samples\n80694-2-RR targets Phospho-YAP1 (Ser127) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated HepG2 cells\nPositive FC (Intra) detected in: Calyculin A treated HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1\/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1\/TAZ. Moreover, phosphorylated YAP1\/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Yes-associated protein 1, 65kDa\nCalculated Molecular Weight: 504 aa, 54 kDa\nObserved Molecular Weight: 70-78 kDa\nGenBank Accession Number: BC038235\nGene Symbol: YAP1\nGene ID (NCBI): 10413\nRRID: AB_3670484\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P46937\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888535064745,"sku":"80694-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-80694-2-rr","provider":"Iright","version":"1.0","type":"link"}