{"product_id":"proteintech-81212-2-rr","title":"Proteintech, 81212-2-RR, Phospho-p38 MAPK (Thr180\/Tyr182) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-p38 MAPK (Thr180\/Tyr182) (81212-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-p38 MAPK (Thr180\/Tyr182) in WB, IHC, ELISA applications with reactivity to human, mouse samples\n81212-2-RR targets Phospho-p38 MAPK (Thr180\/Tyr182) in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Anisomycin treated HeLa cells, UV treated NIH\/3T3 cells,  Anisomycin treated NIH\/3T3 cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nA stress-activated serine\/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: mitogen-activated protein kinase 14\nCalculated Molecular Weight: 360 aa, 41 kDa\nObserved Molecular Weight: 38-42 kDa\nGenBank Accession Number: BC031574\nGene Symbol: p38 MAPK\nGene ID (NCBI): 1432\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q16539\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888685863081,"sku":"81212-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-81212-2-rr","provider":"Iright","version":"1.0","type":"link"}