{"product_id":"proteintech-82485-1-rr","title":"Proteintech, 82485-1-RR, KIF5B Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe KIF5B (82485-1-RR) by Proteintech is a Recombinant antibody targeting KIF5B in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n82485-1-RR targets KIF5B in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  A549 cells,  Jurkat cells,  mouse heart tissue,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human ovarian  cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag16250 Product name: Recombinant human KIF5B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 662-782 aa of BC126279 Sequence: ESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDL Predict reactive species\nFull Name: kinesin family member 5B\nCalculated Molecular Weight: 963 aa, 110 kDa\nObserved Molecular Weight: 110-120 kDa\nGenBank Accession Number: BC126279\nGene Symbol: KIF5B\nGene ID (NCBI): 3799\nRRID: AB_3086482\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P33176\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888432566441,"sku":"82485-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-82485-1-rr","provider":"Iright","version":"1.0","type":"link"}