{"product_id":"proteintech-82730-10-rr","title":"Proteintech, 82730-10-RR, IRS1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe IRS1 (82730-10-RR) by Proteintech is a Recombinant antibody targeting IRS1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n82730-10-RR targets IRS1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, A431 cells,  HEK-293 cells,  HepG2 cells\nPositive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIns receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag11714 Product name: Recombinant human IRS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 895-1243 aa of BC053895 Sequence: EYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ Predict reactive species\nFull Name: insulin receptor substrate 1\nCalculated Molecular Weight: 1242 aa, 132 kDa\nObserved Molecular Weight: 160-185 kDa\nGenBank Accession Number: BC053895\nGene Symbol: IRS1\nGene ID (NCBI): 3667\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P35568\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888772763817,"sku":"82730-10-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-82730-10-rr","provider":"Iright","version":"1.0","type":"link"}