{"product_id":"proteintech-82776-8-rr","title":"Proteintech, 82776-8-RR, Phospho-ULK1 (Ser758) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-ULK1 (Ser758) (82776-8-RR) by Proteintech is a Recombinant antibody targeting Phospho-ULK1 (Ser758) in WB, ELISA applications with reactivity to human samples\n82776-8-RR targets Phospho-ULK1 (Ser758) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IGF-1 treated MCF-7 cells, PC-3 cells,  λ phosphatase treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nUnc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: unc-51-like kinase 1 (C. elegans)\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: NM_003565\nGene Symbol: ULK1\nGene ID (NCBI): 8408\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: O75385\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888805105833,"sku":"82776-8-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-82776-8-rr","provider":"Iright","version":"1.0","type":"link"}