{"product_id":"proteintech-82844-2-rr","title":"Proteintech, 82844-2-RR, Septin 9 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Septin 9 (82844-2-RR) by Proteintech is a Recombinant antibody targeting Septin 9 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n82844-2-RR targets Septin 9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HepG2 cells,  mouse brain tissue,  mouse spleen tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human colon tissue, human colon  cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag1218 Product name: Recombinant human SEPT9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 250-568 aa of BC021192 Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM Predict reactive species\nFull Name: septin 9\nCalculated Molecular Weight: 64 kDa\nObserved Molecular Weight: 65-80 kDa, 38-40 kDa\nGenBank Accession Number: BC021192\nGene Symbol: SEPT9\nGene ID (NCBI): 10801\nRRID: AB_3670573\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9UHD8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888361001129,"sku":"82844-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-82844-2-rr","provider":"Iright","version":"1.0","type":"link"}