{"product_id":"proteintech-83178-2-rr","title":"Proteintech, 83178-2-RR, N4BP3 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe N4BP3 (83178-2-RR) by Proteintech is a Recombinant antibody targeting N4BP3 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples\n83178-2-RR targets N4BP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nN4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag10194 Product name: Recombinant human N4BP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 198-544 aa of BC053323 Sequence: GRSPGTGPSPFSSSLGHLNHLGGSLDRASQGPKEAGPPAVLSCLPEPPPPYEFSCSSAEEMGAVLPETCEELKRGLGDEDGSNPFTQVLEERQRLWLAELKRLYVERLHEVTQKAERSERNLQLQLFMAQQEQRRLRKELRAQQGLAPEPRAPGTLPEADPSARPEEEARWEVCQKTAEISLLKQQLREAQAELAQKLAEIFSLKTQLRGSRAQAQAQDAELVRLREAVRSLQEQAPREEAPGSCETDDCKSRGLLGEAGGSEARDSAEQLRAELLQERLRGQEQALRFEQERRTWQEEKERVLRYQREIQGGYMDMYRRNQALEQELRALREPPTPWSPRLESSKI Predict reactive species\nFull Name: Nedd4 binding protein 3\nCalculated Molecular Weight: 544 aa, 60 kDa\nObserved Molecular Weight: 65-70 kDa\nGenBank Accession Number: BC053323\nGene Symbol: N4BP3\nGene ID (NCBI): 23138\nRRID: AB_3670871\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O15049\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888357822633,"sku":"83178-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-83178-2-rr","provider":"Iright","version":"1.0","type":"link"}