{"product_id":"proteintech-83287-3-rr","title":"Proteintech, 83287-3-RR, DDX47 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe DDX47 (83287-3-RR) by Proteintech is a Recombinant antibody targeting DDX47 in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n83287-3-RR targets DDX47 in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  Jurkat cells,  SH-SY5Y cells,  HEK-293 cells\nPositive IF\/ICC detected in: U2OS cells\nPositive FC (Intra) detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag11953 Product name: Recombinant human DDX47 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 202-455 aa of BC068009 Sequence: FLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR Predict reactive species\nFull Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47\nCalculated Molecular Weight: 455 aa, 51 kDa\nObserved Molecular Weight: 45 kDa\nGenBank Accession Number: BC068009\nGene Symbol: DDX47\nGene ID (NCBI): 51202\nRRID: AB_3670953\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q9H0S4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888419131561,"sku":"83287-3-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-83287-3-rr","provider":"Iright","version":"1.0","type":"link"}