{"product_id":"proteintech-83443-6-rr","title":"Proteintech, 83443-6-RR, NEDD1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NEDD1 (83443-6-RR) by Proteintech is a Recombinant antibody targeting NEDD1 in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human samples\n83443-6-RR targets NEDD1 in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells, HEK-293 cells,  HeLa cells,  Jurkat cells,  MCF-7 cells\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: A431 cells, HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag33603 Product name: Recombinant human NEDD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-332 aa of BC027605 Sequence: MQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAAS Predict reactive species\nFull Name: neural precursor cell expressed, developmentally down-regulated 1\nCalculated Molecular Weight: 72 kDa\nObserved Molecular Weight: 71-75 kDa\nGenBank Accession Number: BC027605\nGene Symbol: NEDD1\nGene ID (NCBI): 121441\nRRID: AB_3671084\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: Q8NHV4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888436793513,"sku":"83443-6-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-83443-6-rr","provider":"Iright","version":"1.0","type":"link"}