{"product_id":"proteintech-83840-7-rr","title":"Proteintech, 83840-7-RR, Phospho-PKC Alpha (Ser657) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-PKC Alpha (Ser657) (83840-7-RR) by Proteintech is a Recombinant antibody targeting Phospho-PKC Alpha (Ser657) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n83840-7-RR targets Phospho-PKC Alpha (Ser657) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated PC-3 cells, PMA treated NIH\/3T3 cells,  Calyculin A treated HSC-T6 cells\nPositive IF\/ICC detected in: NIH\/3T3 cells\nPositive FC (Intra) detected in: Calyculin A treated PC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPKCs are a family of serine\/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ\/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: protein kinase C, alpha\nCalculated Molecular Weight: 77 kDa\nObserved Molecular Weight: 77 kDa\nGenBank Accession Number: AK055431\nGene Symbol: PKC Alpha\nGene ID (NCBI): 5578\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P17252\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888804057257,"sku":"83840-7-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-83840-7-rr","provider":"Iright","version":"1.0","type":"link"}