{"product_id":"proteintech-84654-4-rr","title":"Proteintech, 84654-4-RR, NEK4 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NEK4 (84654-4-RR) by Proteintech is a Recombinant antibody targeting NEK4 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples\n84654-4-RR targets NEK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HEK-293T cells,  HeLa cells,  NCI-H1299 cells\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse testis tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)-P: IF-P : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNeks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine\/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag34929 Product name: Recombinant human NEK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 429-779 aa of BC063044 Sequence: SSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYR Predict reactive species\nFull Name: NIMA (never in mitosis gene a)-related kinase 4\nCalculated Molecular Weight: 781 aa, 88 kDa\nObserved Molecular Weight: 95-100 kDa\nGenBank Accession Number: BC063044\nGene Symbol: NEK4\nGene ID (NCBI): 6787\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P51957\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888358150313,"sku":"84654-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-84654-4-rr","provider":"Iright","version":"1.0","type":"link"}