{"product_id":"proteintech-84974-1-rr","title":"Proteintech, 84974-1-RR, Phospho-POLR2A (Ser2) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-POLR2A (Ser2) (84974-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples\n84974-1-RR targets Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293 cells\nPositive FC (Intra) detected in: λ phosphatase treated HEK-293 cells\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used,  RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity.  Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short.  For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa\nCalculated Molecular Weight: 1970 aa, 217 kDa\nObserved Molecular Weight: 250 kDa\nGenBank Accession Number: BC137231\nGene Symbol: POLR2A\nGene ID (NCBI): 5430\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P24928\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888804155561,"sku":"84974-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-84974-1-rr","provider":"Iright","version":"1.0","type":"link"}