{"product_id":"proteintech-84981-1-rr","title":"Proteintech, 84981-1-RR, Phospho-EIF4E (Ser209) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-EIF4E (Ser209) (84981-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EIF4E (Ser209) in WB, FC (Intra), ELISA applications with reactivity to human samples\n84981-1-RR targets Phospho-EIF4E (Ser209) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Calyculin A treated HEK-293T cells\nPositive FC (Intra) detected in: Calyculin A treated HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: eukaryotic translation initiation factor 4E\nCalculated Molecular Weight: 29 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC012611\nGene Symbol: EIF4E\nGene ID (NCBI): 1977\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nUNIPROT ID: P06730\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888801140905,"sku":"84981-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-84981-1-rr","provider":"Iright","version":"1.0","type":"link"}