{"product_id":"proteintech-85141-4-rr","title":"Proteintech, 85141-4-RR, NDRG1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe NDRG1 (85141-4-RR) by Proteintech is a Recombinant antibody targeting NDRG1 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n85141-4-RR targets NDRG1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  A431 cells,  A549 cells,  mouse kidney tissue,  mouse brain tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha\/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag25424 Product name: Recombinant human NDRG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 45-109 aa of BC006260 Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC Predict reactive species\nFull Name: N-myc downstream regulated 1\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 45-46 kDa\nGenBank Accession Number: BC006260\nGene Symbol: NDRG1\nGene ID (NCBI): 10397\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q92597\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888652275881,"sku":"85141-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-85141-4-rr","provider":"Iright","version":"1.0","type":"link"}