{"product_id":"proteintech-85753-1-rr","title":"Proteintech, 85753-1-RR, Phospho-IRAK4 (Thr345\/Ser346) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-IRAK4 (Thr345\/Ser346) (85753-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-IRAK4 (Thr345\/Ser346) in WB, IHC, ELISA applications with reactivity to human, rat samples\n85753-1-RR targets Phospho-IRAK4 (Thr345\/Ser346) in WB, IHC, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: IL-1 beta treated Karpas-299 cells\nPositive IHC detected in: rat lymph node tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: interleukin-1 receptor-associated kinase 4\nCalculated Molecular Weight: 52 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC013316\nGene Symbol: IRAK4\nGene ID (NCBI): 51135\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9NWZ3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888802287785,"sku":"85753-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-85753-1-rr","provider":"Iright","version":"1.0","type":"link"}