{"product_id":"proteintech-86077-2-rr","title":"Proteintech, 86077-2-RR, CHD3 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe CHD3 (86077-2-RR) by Proteintech is a Recombinant antibody targeting CHD3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n86077-2-RR targets CHD3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  MCF-7 cells,  Raji cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: chromodomain helicase DNA binding protein 3\nCalculated Molecular Weight: 227 kDa\nObserved Molecular Weight: 250 kDa\nGenBank Accession Number: NM_005852\nGene Symbol: CHD3\nGene ID (NCBI): 1107\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q12873\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888725741737,"sku":"86077-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-86077-2-rr","provider":"Iright","version":"1.0","type":"link"}