{"product_id":"proteintech-86238-2-rr","title":"Proteintech, 86238-2-RR, Pan-Keratin (Type I) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Pan-Keratin (Type I) (86238-2-RR) by Proteintech is a Recombinant antibody targeting Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n86238-2-RR targets Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HaCaT cells, A431 cells,  HT-29 cells,  mouse skin tissue,  rat skin tissue,  rat colon tissue\nPositive IHC detected in: human prostate cancer tissue, Human Kidney(renal cell) Cancer,  human tonsil tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human cervical cancer tissue, human colon cancer tissue,  human lung cancer tissue,  mouse skin tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)-P: IF-P : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKeratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic\/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: Pan-Keratin (Type I)\nObserved Molecular Weight: 45-65 kDa\nGene ID (NCBI): Pan-Keratin (Type I)\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q15323\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888795963561,"sku":"86238-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-86238-2-rr","provider":"Iright","version":"1.0","type":"link"}