{"product_id":"proteintech-86645-1-rr","title":"Proteintech, 86645-1-RR, E2F8 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe E2F8 (86645-1-RR) by Proteintech is a Recombinant antibody targeting E2F8 in WB, IP, ELISA applications with reactivity to human samples\n86645-1-RR targets E2F8 in WB, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293T cells,  Jurkat cells\nPositive IP detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nE2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. \nThe calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag4216 Product name: Recombinant human E2F8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 78-508 aa of BC028244 Sequence: MAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTDATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLGTLFVPQRKLEVSTEDVH Predict reactive species\nFull Name: E2F transcription factor 8\nCalculated Molecular Weight: 867 aa, 94 kDa\nObserved Molecular Weight: 105 kDa\nGenBank Accession Number: BC028244\nGene Symbol: E2F8\nGene ID (NCBI): 79733\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: A0AVK6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888612069545,"sku":"86645-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-86645-1-rr","provider":"Iright","version":"1.0","type":"link"}