{"product_id":"proteintech-86728-1-rr","title":"Proteintech, 86728-1-RR, TRIM47 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe TRIM47 (86728-1-RR) by Proteintech is a Recombinant antibody targeting TRIM47 in WB, ELISA applications with reactivity to human, mouse, rat samples\n86728-1-RR targets TRIM47 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse colon tissue,  OVCAR-3 cells,  U2OS cells,  SMMC-7721 cells,  PC-3 cells,  rat colon tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTRIM47\/GOA is a member of the tripartite motif RBCC\/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47\/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag22868 Product name: Recombinant human TRIM47 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017299 Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR Predict reactive species\nFull Name: tripartite motif-containing 47\nCalculated Molecular Weight: 638 aa, 70 kDa\nObserved Molecular Weight: 70-75 kDa\nGenBank Accession Number: BC017299\nGene Symbol: TRIM47\nGene ID (NCBI): 91107\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q96LD4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888836333737,"sku":"86728-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-86728-1-rr","provider":"Iright","version":"1.0","type":"link"}