{"product_id":"proteintech-86990-2-rr","title":"Proteintech, 86990-2-RR, Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Di-Methyl-Histone H3 (Lys9) (86990-2-RR) by Proteintech is a Recombinant antibody targeting Di-Methyl-Histone H3 (Lys9) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human samples\n86990-2-RR targets Di-Methyl-Histone H3 (Lys9) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293T cells\nPositive IF\/ICC detected in: HeLa cells\nPositive Dot Blot detected in: \/ \/\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:1250-1:5000\nDOT BLOT: DOT BLOT : 1:10-1:100\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nH3K9me2 is a typical transcriptional repressive modification, primarily enriched in heterochromatin regions (such as centromeres, telomeres, and transposon elements), but can also be scattered throughout euchromatin. By recruiting \"reader\" proteins such as HP1 (Heterochromatin Protein 1), H3K9me2 can establish and stabilize a chromatin condensation state, thereby silencing gene expression.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 1, H3a\nObserved Molecular Weight: 15-17 kDa\nGenBank Accession Number: BC066245\nGene Symbol: HIST1H3A\nGene ID (NCBI): 8350\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P68431\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888736293033,"sku":"86990-2-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-86990-2-rr","provider":"Iright","version":"1.0","type":"link"}