{"product_id":"proteintech-87220-1-rr","title":"Proteintech, 87220-1-RR, Phospho-AKT (Thr450) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-AKT (Thr450) (87220-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-AKT (Thr450) in WB, ELISA applications with reactivity to human samples\n87220-1-RR targets Phospho-AKT (Thr450) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, λ phosphatase treated MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: v-akt murine thymoma viral oncogene homolog 1\nCalculated Molecular Weight: 56 kDa\nObserved Molecular Weight: 60 kDa\nGenBank Accession Number: NM_005163\nGene Symbol: AKT1\nGene ID (NCBI): 207\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P31749\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888799338665,"sku":"87220-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-87220-1-rr","provider":"Iright","version":"1.0","type":"link"}