{"product_id":"proteintech-87465-1-rr","title":"Proteintech, 87465-1-RR, Integrin beta 5 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Integrin beta 5 (87465-1-RR) by Proteintech is a Recombinant antibody targeting Integrin beta 5 in WB, IF\/ICC, ELISA applications with reactivity to mouse, rat samples\n87465-1-RR targets Integrin beta 5 in WB, IF\/ICC, ELISA applications and shows reactivity with mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Neuro-2a cells, C6 cells,  mouse lung tissue\nPositive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: RAW 264.7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIntegrin beta-5 (ITGB5) is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Eg10456 Product name: Recombinant mouse ITB5 protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Domain: 10011459 of Sequence: \\ Predict reactive species\nFull Name: integrin beta 5\nObserved Molecular Weight: 100 kDa\nGene Symbol: Itgb5\nGene ID (NCBI): 16419\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888771322025,"sku":"87465-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-87465-1-rr","provider":"Iright","version":"1.0","type":"link"}