{"product_id":"proteintech-87523-1-rr","title":"Proteintech, 87523-1-RR, Phospho-CDK9 (Thr186) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CDK9 (Thr186) (87523-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CDK9 (Thr186) in WB, ELISA applications with reactivity to human, mouse, rat samples\n87523-1-RR targets Phospho-CDK9 (Thr186) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. Phospho-CDK9 (Thr186) is the active-site-switching modification of the catalytic subunit of P-TEFb (positive transcription elongation factor b). Phosphorylation at threonine 186, located in the T-loop of CDK9, is essential for full kinase activity: it swings the T-loop away from the catalytic cleft, allowing ATP and protein substrates (e.g., the C-terminal domain of RNA polymerase II, DSIF and NELF) to enter and be efficiently phosphorylated. Thus, p-Thr186-CDK9 licenses the elongation step of most protein-coding transcripts and is indispensable for productive mRNA synthesis. (PMID: 39800748)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cyclin-dependent kinase 9\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 42 kDa\nGenBank Accession Number: NM_001261\nGene Symbol: CDK9\nGene ID (NCBI): 1025\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P50750\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888800223401,"sku":"87523-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-87523-1-rr","provider":"Iright","version":"1.0","type":"link"}