{"product_id":"qiagen-215011","title":"Qiagen, 215011, FastLane Cell cDNA Kit (50)\nicon_0368_ls_gen_eco_friendly-s","description":"\u003cp\u003eBuffer FCW, Buffer FCP, and components for 50 x 20 µl reverse-transcription reactions (gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, and RNase-Free Water)\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003c\/p\u003e\n\n\u003cp\u003e- From cells to cDNA in only 4 steps and under 45 minutes\u003cbr\u003e\n- Sensitive detection of even low-abundance transcripts\u003cbr\u003e\n- Easy parallel processing of several samples\u003cbr\u003e\n- Integrated gDNA removal allows detection of RNA only\u003cbr\u003e\n- No need to design RNA-specific primers or probes\u003c\/p\u003e\n\n\u003cp\u003eProduct Details\u003c\/p\u003e\n\n\u003cp\u003eThe FastLane Cell cDNA Kit provides a fast and simple procedure for preparing first-strand cDNA directly from cultured cells without RNA purification. The kit is supplied with wash and lysis buffers for preparing lysates and stabilizing RNA, gDNA Wipeout Buffer for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis. The FastLane Cell cDNA Kit is ideal for experiments requiring rapid, high-throughput gene expression analysis.\u003c\/p\u003e\n\n\u003cp\u003ePrinciple\u003c\/p\u003e\n\n\u003cp\u003eThe FastLane Cell cDNA Kit uses FastLane technology to prepare cDNA directly from cultured cells in just 45 minutes. To ensure only RNA is detected in real-time RT-PCR, novel gDNA Wipeout Buffer is used to eliminate genomic DNA (see figure \" Effective elimination of genomic DNA contamination \"). Time and effort are saved as there is no need to design RNA-specific primers or probes.\u003c\/p\u003e\n\n\u003cp\u003eWhen the FastLane Cell cDNA Kit is used together with optimized QuantiTect, QuantiFast, or Rotor-Gene Kits for real-time RT-PCR, several cell samples can be easily processed and analyzed within a few hours. This allows a snapshot of several transcripts in experiments such as validation of siRNA-mediated gene knockdown (see figure \" Successful analysis of CDC2 knockdown \").\u003c\/p\u003e\n\n\u003cp\u003eThe FastLane Cell cDNA Kit is part of the comprehensive range of FastLane Kits, which speed up and streamline the gene expression analysis workflow.\u003c\/p\u003e\n\n\u003cp\u003eProcedure\u003c\/p\u003e\n\n\u003cp\u003e- SYBR ® Green detection: QuantiTect SYBR ® Green PCR Kit\u003cbr\u003e\n- Probe detection: QuantiTect Probe PCR Kit\u003cbr\u003e\n- Multiplex probe detection: QuantiTect Multiplex PCR Kits\u003c\/p\u003e\n\n\u003cp\u003e- SYBR ® Green detection: QuantiFast SYBR ® Green PCR Kit\u003cbr\u003e\n- Probe detection: QuantiFast Probe PCR Kits\u003cbr\u003e\n- Multiplex probe detection: QuantiFast Multiplex PCR Kits\u003c\/p\u003e\n\n\u003cp\u003e- SYBR ® Green detection: Rotor-Gene SYBR ® Green PCR Kit\u003cbr\u003e\n- Probe detection: Rotor-Gene Probe PCR Kit\u003cbr\u003e\n- Multiplex probe detection: Rotor-Gene Multiplex PCR Kit\u003c\/p\u003e\n\n\u003cp\u003eWith the FastLane Cell cDNA Kit, cDNA is obtained from cultured cells in just 45 minutes in a 4-step procedure: cell wash; cell lysis with integrated RNA stabilization; genomic DNA elimination; and cDNA synthesis. The synthesized cDNA is ready to use in real-time two-step RT-PCR.\u003c\/p\u003e\n\n\u003cp\u003eThe kit contains sufficient reagents for preparing cDNA from two 24-well cell-culture plates or one 48-well cell-culture plate. If working with a 96-well cell-culture plate, an additional QuantiTect Reverse Transcription Kit (50) needs to be purchased.\u003c\/p\u003e\n\n\u003cp\u003ecDNA prepared with the FastLane Cell cDNA Kit can be analyzed by real-time two-step RT-PCR using one of the following QuantiTect Kits:\u003c\/p\u003e\n\n\u003cp\u003eAlternatively, fast real-time two-step RT-PCR can be carried out when the FastLane Cell cDNA Kit is used in combination with a QuantiFast Kit:\u003c\/p\u003e\n\n\u003cp\u003eUltrafast real-time two-step RT-PCR can be achieved when cDNA prepared with the FastLane Cell cDNA Kit is analyzed on the Rotor-Gene Q cycler using one of the following dedicated Rotor-Gene Kits:\u003c\/p\u003e\n\n\u003cp\u003eQuantiTect and QuantiFast Kits are compatible with various real-time cyclers, including instruments from Applied Biosystems.\u003c\/p\u003e\n\n\u003cp\u003eApplications\u003c\/p\u003e\n\n\u003cp\u003e- Validation of siRNA-mediated gene knockdown\u003cbr\u003e\n- Evaluation of drug effects\u003cbr\u003e\n- Detection of gene regulation\u003c\/p\u003e\n\n\u003cp\u003eThe FastLane Cell cDNA Kit is suitable for experiments which require a snapshot of individual transcript levels, such as:\u003c\/p\u003e","brand":"Qiagen","offers":[{"title":"Default Title","offer_id":46646771089577,"sku":"215011","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/qiagen-215011","provider":"Iright","version":"1.0","type":"link"}