{"product_id":"revvity-61fgbdlf","title":"Revvity, 61FGBDLF, HTRF Anti-FLAG mAb d2-Conjugate, 1,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nMAb Anti FLAG-d2 is an antibody raised against FLAG® fusion proteins labeled with d2. The anti-FLAG antibody will recognize the FLAG sequence at the N-terminus, intra, or C-terminus of FLAG fusion proteins.\u003cbr\u003e\nThis reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein\/protein, protein\/peptide, protein\/DNA, protein\/RNA, protein\/carbohydrate, protein\/small molecule, and receptor\/ligand.\u003cbr\u003e\nHTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nIn an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the resulting signal is proportional to the binding of the 2 partners. In the example shown here, MAb Anti FLAG-d2 binds to the FLAG tagged partner A, while partner B* binds to a specific Ab labeled with an HTRF donor. \u003cbr\u003e\n*partner B can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with a donor for the detection.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a FLAG-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add MAb Anti FLAG-d2 (5 µL) and anti-partner B labeled with donor (5 µL), then incubate and read. \u003cbr\u003e\n*partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with a donor for the detection. Application-Protein-Protein Interaction\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-1,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775288627369,"sku":"61FGBDLF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-61fgbdlf","provider":"Iright","version":"1.0","type":"link"}