{"product_id":"revvity-61gt0bla","title":"Revvity, 61GT0BLA, HTRF Anti-PolyGT Biotin Antibody, 5,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPoly-(Glu,Tyr) (4:1) is a random copolymer which has been shown to be a substrate for tyrosine-specific protein kinases. Poly-(GT)-biotin may be used with Streptavidin-XL665 and Anti-phosphotyrosine-cryptate for easily optimized HTRF® tyrosine kinase assays. HTRF enables to perform assay at physiological ATP concentrations.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principles\u003cbr\u003e\nHTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and PolyGT-Biotin as substrate. The signal is proportional to the concentration of phospho-tyrosine.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nHTRF tyrosine kinase assay have two basic phases: 1. Enzymatic step: biotinylated PolyGT is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated PolyGT is then detected by the addition of Streptavidin-XL665 and a Europium-cryptate labeled anti-phosphotyrosine antibody (MAb PY20-Eu cryptate or MAb PT66-Eu cryptate) prepared in a buffer containing EDTA to stop the enzymatic reaction. Application-Biochemical Enzymatic Assay\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTarget Class-Kinases\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Inflammation Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-5,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775300620457,"sku":"61GT0BLA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-61gt0bla","provider":"Iright","version":"1.0","type":"link"}