{"product_id":"revvity-61histlf","title":"Revvity, 61HISTLF, HTRF Anti-6His mAb Tb-Conjugate, 1,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nMAb Anti-6HIS-Tb cryptate is an IgG2a raised against a polyhistidine tagged fusion protein labeled with Tb. It recognizes synthetic polyhistidine or polyhistidine-tagged fusion protein.\u003cbr\u003e\nThis reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein\/protein, protein\/peptide, protein\/DNA, protein\/RNA, protein\/carbohydrate, protein\/small molecule, receptor\/ligand.\u003cbr\u003e\nHTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nIn an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here: MAb Anti 6HIS-Tb cryptate binds to the 6 His tagged partner A while partner B* binds to a specific Ab labeled with an HTRF acceptor. *partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a 6 His-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add MAb Anti 6HIS-Tb cryptate (5 µL) and anti-partner B labeled with acceptor. (5 µL), incubate and read. *partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases use for the detection, the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin), labeled with acceptor. Application-Protein-Protein Interaction\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTarget Class-Binding Assay\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Inflammation Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-1,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775287775401,"sku":"61HISTLF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-61histlf","provider":"Iright","version":"1.0","type":"link"}