{"product_id":"revvity-62bdbar2peb","title":"Revvity, 62BDBAR2PEB, HTRF β-arrestin 2 Recruitment Kit, 1,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe B-arr2 recruitment cellular assay monitors the recruitment of Beta-arrestin 2 and AP2 through GPCR activation. The B-arr2 recruitment kit is used to characterize the effect of compounds on the Beta-arrestin signaling pathway, by monitoring the interaction between endogenous B-arrestin 2 and AP-2 as its partner.\u003cbr\u003e\nIt is known from the literature that pharmacological investigations on this interaction are relevant when investigating the Beta-arrestin signaling pathway in a GPCR context.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nB-arr2 recruitment kit assay principle\u003cbr\u003e\nThe B-arr2 recruitment assay monitors the recruitment of B-arrestin 2 mediated by GPCR activation in cells. Revvity's B-arr2 recruitment kit is highly sensitive, enabling the pharmacological characterization of compounds involving endogenous B-arrestin 2 and AP2 proteins. The kit uses two labeled antibodies, one coupled to a donor fluorophore, and the other to an acceptor. Both antibodies are highly specific for B-arr2 protein and AP2. In presence of B-arr2\/AP2 interaction in cells, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the number of B-arrestin 2\/AP2 complexes present in the sample.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eB-arr2 recruitment assay protocol (option 1)\u003cbr\u003e\nDetection of B-arr2 recruitment​​​​​​​ with HTRF reagents can be performed in a single plate used for culturing, stimulation, stabilization, and detection. Three washes are required after the stabilization step. This specific protocol enables detection with non-lysed cells and endogenous proteins after ON incubation at room temperature, while maintaining robust HTRF quality.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eB-arr2 recruitment assay protocol (option 2)\u003cbr\u003e\nDetection of B-arr2 recruitment​​​​​​​ with HTRF reagents can be performed in a single plate used for culturing, stimulation, stabilization and detection. Three washes are required after the stabilization step. This specific protocol enables detection with non-lysed cells and endogenous proteins, while maintaining robust HTRF quality after ON incubation at room temperature.\u003cbr\u003e\nOption 2 of the B-arr2 recruitment assay protocol enables improvement of S\/B by removing 80µL of detection reagents after ON incubation at room temperature. Application-Second Messenger Detection\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-200 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-GPCR\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Inflammation Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-1,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775238656169,"sku":"62BDBAR2PEB","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62bdbar2peb","provider":"Iright","version":"1.0","type":"link"}