{"product_id":"revvity-62cxcl12peh","title":"Revvity, 62CXCL12PEH, HTRF Human and Mouse CXCL12 Detection Kit, 10,000 assay points","description":"\u003cp\u003eOverview\u003cbr\u003e\nCXCL12 (SDF-1) is a homeostatic chemokine constitutively expressed by bone marrow stromal cells, and is present in many other tissues (skin, thymus, lymph nodes, lung, liver). It plays an essential role in the homeostatic regulation of leukocyte traffic, hematopoiesis, organogenesis, cell differentiation, and tissue regeneration.\u003cbr\u003e\nSDF-1 binds to and activates the CXCR4 receptor. CXCL12 and CXCR4 are overexpressed in various cancer types, and this aberrant expression strongly promotes proliferation, migration, and invasion through multiple signal pathways.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the HTRF CXCL12 assay\u003c\/p\u003e\n\n\n\u003cp\u003eThe human and mouse CXCL12 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to CXCL12, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of CXCL12 present in the sample.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\u003cp\u003eProtocol of the HTRF CXCL12 assay\u003c\/p\u003e\n\n\n\u003cp\u003eCell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF™ reagents (384-well low-volume white plate or Revvity low-volume 96-well plate, in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nDynamic Range-93.2 – 8000 pg\/mL\u003cbr\u003e\nLimit of Detection-1.8 pg\/mL\u003cbr\u003e\nLimit of Quantification-7.3 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-CXCL12\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775264379049,"sku":"62CXCL12PEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62cxcl12peh","provider":"Iright","version":"1.0","type":"link"}