{"product_id":"revvity-62d2dpea","title":"Revvity, 62D2DPEA, HTRF d2 Labeling Kit","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe d2 labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with d2.\u003cbr\u003e\nThis kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRF assay development.\u003cbr\u003e\nThe N-hydroxysuccinimide activated d2 supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.\u003cbr\u003e\nThe kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nThe labeling procedure is performed under very mild conditions. As shown here, the process comprises an incubation step with the desiccated dye, followed by purification on a disposable column. Both the purification column and the buffer for purifying the conjugate are included in the kit, as well as a calibrator to define the ideal working dilution of the labeled material in HTRF assays. Depending on the molecular weight of the target molecule and the nature of the dye, each kit enables the labeling of 5-25 µg (protein-MW 15 kDa) to 50-250 µg (antibody-MW 150 kDa).\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe labeling procedure is described on the right. 100 µL of the targeted molecule (16.67 µM) in carbonate buffer pH 9.0 are added to the d2 vial. After an incubation of 45 minutes at RT, labeled protein is eluted on an equilibrated column. After having discarded 700 µL of elution buffer, the labeled molecule is collected in 400µL fraction. Application-Labeling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-1 Kit\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775244816553,"sku":"62D2DPEA","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62d2dpea","provider":"Iright","version":"1.0","type":"link"}