{"product_id":"revvity-62estpeg","title":"Revvity, 62ESTPEG, HTRF Estradiol Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThere is substantial research indicating that breast cancer tissue contains the enzymes necessary to synthesize estradiol (17ß-estradiol), a steroid hormone that plays an important role in this disease. Two main pathways are involved in the formation of estradiol in breast cancer tissues: the aromatase pathway, which transforms androgens into estrogens, and the sulfatase pathway, which converts estrone into estradiol via the 17ß-HSD1 enzyme. The Estradiol kit is a highly specific assay for measuring estradiol in cell supernatants, or biochemical enzyme assays.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nThe kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and estradiol coupled to XL665 (acceptor). Estradiol produced by cells or purified 17ß-HSD1 enzyme competes with XL665-labelled estradiol for binding to monoclonal anti-estradiol Eu Cryptate.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe estradiol assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Estradiol detection using HTRF reagents This protocol requires only a single, two-hour incubation period following cell stimulation. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-10 µL\u003cbr\u003e\nShipping Conditions-Shipped Ambient\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Metabolism\/Diabetes\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775247536297,"sku":"62ESTPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62estpeg","provider":"Iright","version":"1.0","type":"link"}