{"product_id":"revvity-62hil22peg","title":"Revvity, 62HIL22PEG, HTRF Human IL-22 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nInterleukin 22 is a type 2 cytokine (member of the IL10 family) and plays an essential role in innate protective immunity against extracellular pathogens. It induces pro-inflammatory responses, allows production of antimicrobial peptides. IL-22 is also involved in wound-healing and regeneration of epithelial cells. Human IL-22 is produced on inflammation site by Th17 CD4+ cells, activated T-cells, Th1 and NK cells. After being secreted, it targets non-hematopoietic cells and binds to IL22R that is restricted to tissue-resident cells (fibroblasts, epithelial cells). Downstream signaling involves phosphorylation of Jak1 and Tyk2 and subsequent activation of STAT3. Cisbio’s Human IL22 kit is a homogeneous, add and read assay to measure IL-22 secretion in cell supernatants. It offers a fast, efficient alternative to ELISA.\u003cbr\u003e\nAssessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay Principle\u003cbr\u003e\nCell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µL). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.\u003cbr\u003e\n \u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\u003cp\u003eAssay data analysis\u003cbr\u003e\nThe 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.\u003cbr\u003e\nTo fully understand how to deal with HTRF data processing, and also 4PL 1\/y² fitting.\u003cbr\u003e\nRevvity also works with Myassays.com to help you in your data analysis. You’ll be able to access a free online software to run your IL22 analysis. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775277748393,"sku":"62HIL22PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62hil22peg","provider":"Iright","version":"1.0","type":"link"}