{"product_id":"revvity-62hmgpeg","title":"Revvity, 62HMGPEG, HTRF HMGB1 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nIn the nucleus, ​High-mobility group box 1 (HMGB1) interacts with DNA and histones to determine chromatin structure. Once released, HMGB1 acquires a new role as an alarmin or a damage-associated molecular pattern (DAMP) and is capable of initiating a state of inflammation by binding TLR4 and promoting the production of cytokines.\u003cbr\u003e\nNecrosis, pyroptosis, and apoptosis are mechanisms of regulated cell death that have been shown to lead to the release of different forms of HMGB1. In pyroptosis, HMGB1 is released together with IL-1b and IL-18.\u003cbr\u003e\nHMGB1 is implicated in the pathology of several auto-immune diseases, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE).\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nHMGB1 is measured using a sandwich immunoassay involving two specific anti-HMGB1 antibodies, respectively labeled with an HTRF donor and acceptor dyes. The intensity of the signal is directly proportional to the concentration of HMGB1 present in the sample.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay principle\u003cbr\u003e\nThe assay protocol, using a 384-well small volume white plate or a Revvity low volume 96-well plate (20 µL final), is described on the right. 16µl of cell supernatant, sample, or standard are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Inflammation Metabolism\/Diabetes NASH\/Fibrosis Oncology \u0026amp; Inflammation\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775265296553,"sku":"62HMGPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62hmgpeg","provider":"Iright","version":"1.0","type":"link"}