{"product_id":"revvity-62hpil1bpeg","title":"Revvity, 62HPIL1BPEG, HTRF Human pro IL-1β Detection Kit, 500 assay points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPro IL-1β, also known as IL-1β precursor, is a 31 kDa protein precursor of IL-1β. It is produced in response to molecular motifs carried by pathogens called ‘pathogen associated molecular patterns’ (PAMPs). Induction of pro IL-1β expression is referred to as a priming step and is an inefficient secretion stimulus. The processing and cleavage of pro IL-1β into active IL-1β is conditioned by the activation stage involving PAMP, or DAMP (danger associated molecular pattern, endogenous molecules released from dead cells).\u003cbr\u003e\nPro IL-1β is cleaved by the pro-inflammatory protease caspase-1, activated via recruitment to a multi-protein complex termed the inflammasome.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\n Stable signal, providing flexibility in readout time or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nPrinciple of the HTRF human pro IL-1β assay\u003cbr\u003e\nThe HTRF pro IL-1β assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labeled with Eu3+-cryptate (donor) and the other with d2 (acceptor). One antibody is directed against the IL-1β, and the second recognizes specifically pro IL-1β. When both antibodies bind to pro IL-1β, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of pro IL-1β present in the cell lysate sample.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\u003cp\u003eProtocol of the HTRF human pro IL-1β assay\u003cbr\u003e\nThe HTRF pro IL-1β assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples (cell lysates) or standards are dispensed directly into the assay plate for the detection of pro IL-1β by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nDynamic Range-312.5 - 20,000 pg\/mL\u003cbr\u003e\nLimit of Detection-38.5 pg\/mL\u003cbr\u003e\nLimit of Quantification-130 pg\/mL\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget-pro-IL1β\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Autoimmunity Cardiovascular Immuno-oncology Infectious Diseases Inflammation Neuroscience Oncology Virology\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775279780009,"sku":"62HPIL1BPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62hpil1bpeg","provider":"Iright","version":"1.0","type":"link"}