{"product_id":"revvity-62kb02rede","title":"Revvity, 62KB02REDE, HTRF Dasatinib Red Ligand, 20 nmoles","description":"\u003cp\u003eOverview\u003cbr\u003e\nThis compound is derived from Dasatinib, which is a well known inhibitor of the BCR-ABL and SRC families of Kinases. Dasatinib, which is an ATP-competitive Kinase inhibitor, is used as a drug to treat chronic myelogenous leukemia and acute lymphoblastic leukemia. It has been shown to bind with an affinity of \u0026lt;100 nM to about 16% of the Kinases and mainly to RTK. It is therefore useful to address multiple Kinases in the HTRF Kinase Binding assay platform. The ‘d2’ fluorescent dye used absorbs with its maximum near 650 nm and fluoresces near 670 nm. It is used as an acceptor molecule in HTRF assays.\u003cbr\u003e\nDasatinib-Red is supplied as a 25 µM solution in DMSO.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nKd determination: assay principal\u003cbr\u003e\n​The binding of Dasatinib-Red is detected in a sandwich assay format using the Anti Tag labeled with Europium Cryptate (donor) which binds to the tagged Kinase, and a red fluorescent tracer labelled with d2 (acceptor).The detection principle is based on HTRF technology.  The HTRF ratio (665\/620) will increase upon the addition of more of the tracer, and will saturate depending on the dissociation constant (Kd) of the tracer to the tagged kinase. To know whether Dasatinib-Red is the tracer best suited to your tagged kinase of interest, we advise you to try the Kinase Binding Discovery kits.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003cbr\u003e\nKd determination assay protocol\u003cbr\u003e\nSaturation binding experiments of  Dasatinib-Red can be run in 96- or 384-well plates (20 µL final volume).First, a dilution series ranging between 0 and 1  µM of Dasatinib-Red in the Kinase Binding Buffer is prepared in a 96-well non-binding plate. Next, 5 µL of Kinase Binding Buffer are dispensed into the final 96- or 384-well plate. Then 5 µL of tagged-Kinase are added, followed by 5 µL of Anti-tag Eu-cryptate. Finally, 5 µL of the red tracer solution are added. The HTRF ratio is measured after 1 H of incubation.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003cbr\u003e\nIC50\/Ki determination assay principle\u003cbr\u003e\nThe binding of Dasatinib-Red is detected in a sandwich assay format using a specific Anti-GST, 6HIS antibody or Streptavidin labeled with Europium Cryptate (donor), which binds to the tagged Kinase, and a red fluorescent Dasatinib labelled with d2 (acceptor).The detection principle is based on HTRF® technology.  The HTRF ratio (665\/620) will increase upon the addition of more Dasatinib-Red, and will saturate depending on the dissociation constant (Kd ) of Staurosporine-Red to the tagged kinase. With the addition of an inhibitor of the kinase, Dasatinib-Red will be displaced and the HTRF signal will disappear, depending on the dose.\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003cbr\u003e\nIC50\/Ki determination assay protocol\u003cbr\u003e\n​​Pharmacological evaluation of inhibitors of interest can be run in 96- or 384-well plates.\u003cbr\u003e\nFirst, a dilution series of inhibitor ranging between 40 µM and 0.23 nM is prepared, wih 5 µL of each concentration dispensed into the plate. Then 5 µL of tagged-Kinase are added, followed by 5 µL of anti-tag Eu-cryptate. Finally, 5 µL of Dasatinib-Red solution are added, prepared at 4x the final concentration.  The HTRF ratio is measured after 1 H of incubation.\u003cbr\u003e\nAnalyses of the data give typical dose response curves ranging between 10 µM and 56 pM, enabling evaluation of the IC50\/Ki values for the inhibitor of interest. Application-Biochemical Enzymatic Assay\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Kinases\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Metabolism\/Diabetes Neuroscience Oncology \u0026amp; Inflammation\u003cbr\u003e\nUnit Size-20 nmol\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775244914857,"sku":"62KB02REDE","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62kb02rede","provider":"Iright","version":"1.0","type":"link"}