{"product_id":"revvity-62mmp1peg","title":"Revvity, 62MMP1PEG, HTRF Human MMP1 Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nMatrix metalloproteinase (MMP) 1, also known as interstitial collagenase, is an endopeptidase secreted as an inactive proenzyme that is then activated by proteolytic cleavage. MMP1 plays a significant role in tissue repair and remodeling by degrading structural components of the extracellular matrix (ECM), such as collagens, gelatin, and fibronectin. As such it is closely related to all matrix-dependent disorders, such as fibrotic disease, and some types of cancer that rely on cell adhesion, migration, and invasion.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nHuman MMP1 assay principle\u003c\/p\u003e\n\n\n\u003cp\u003eThe Human Matrix MetalloProteinase 1 (MMP1) assay measures MMP1 in cell supernatant. The assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. In presence of Human MMP1 in a sample, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and therefore provides a means of assessing any changes caused by experimental variables, in a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\u003cp\u003eHuman MMP1 assay protocol\u003c\/p\u003e\n\n\n\u003cp\u003eThe Human MMP1 assay protocol, using a 384-well small volume white plate,  is described on the right. 16 µL of sample or standard are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with HTRF fluorophores are added before an overnight incubation. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775266443433,"sku":"62MMP1PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-62mmp1peg","provider":"Iright","version":"1.0","type":"link"}