{"product_id":"revvity-64aav6peh","title":"Revvity, 64AAV6PEH, HTRF AAV6 Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nAdeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 6 (AAV6) is an efficient vector for gene delivery to skeletal muscle or lung tissue. The AAV6 Capsid kit is designed for the quantitative measurement of Adeno-associated virus serotype 6 (AAV6) particles in both cell lysates and cell supernatants.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAAV6 Capsid assay principle\u003cbr\u003e\nThe Adeno-Associated Virus serotype 6 (AAV6) assay measures AAV6 Capsid in cell supernatant or cell lysate. The assay uses two anti-AAV6 antibodies: one coupled to HRP, that binds to anti-HRP d2 (the acceptor) in premix 1, and the other coupled to biotin, that binds to Streptavidin Eu-cryptate (the donor) in premix 2. In presence of AAV6 Capsid in a cell extract or supernatant, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and provides a means of assessing any changes caused by experimental variability under a no-wash assay format.\u003c\/p\u003e\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAAV6 Capsid assay protocol\u003cbr\u003e\nThe AAV6 Capsid assay protocol using a 384-well small volume white plate is described on the right. 5 µL of sample or standard and 5 µL of diluent are dispensed directly into the plate for detection by HTRF® reagents. The Biotin anti-AAV6 antibody is pre-mixed with Streptavidin labeled with the donor, and HRP anti-AAV6 is pre-mixed with anti-HRP labeled with the acceptor. 5 µL of each premix are added. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Bioprocessing\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-5 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Viral Particles\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775234068649,"sku":"64AAV6PEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64aav6peh","provider":"Iright","version":"1.0","type":"link"}