{"product_id":"revvity-64alky04peg","title":"Revvity, 64ALKY04PEG, HTRF Phospho-ALK (Tyr1604) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nALK (anaplastic lymphoma kinase) is a transmembrane tyrosine kinase belonging to the superfamily of insulin receptors. ALK regulates several pathways involved in cell survival, proliferation, and cell cycling, including the AKT\/PI3K,MAPK\/ERK, STAT3 and NFkB pathways.The ALK ligands, pleiotrophin (PTN) and midkine(MDK), induce ALK phosphorylation and activation on  multiple Tyrosine including Y1604. ALK deregulation is associated with  various cancers such as NSCLC, and neuroblastomas. Indeed, oncogenic ALK is driven by i) gene translocations and subsequent intracellular ALK fusion proteins (eg, EML4-ALK, NPM1-ALK, TPM3-ALK, VCL-ALK); ii) mutations (eg G263, R401, R551, P968 and E1242). Oncogenic ALK is constitutive active which results in uncontroled activation of downstream signaling pathways, leading to tumor cells survival and proliferation.\u003cbr\u003e\nHow it works\u003cbr\u003e\nPhospho-ALK (Tyr1604) assay principle\u003cbr\u003e\nThe HTRF Phospho-ALK (Tyr1604) assay measures ALK when phosphorylated at Tyr1604. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-ALK (Tyr1604) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, and the second for its ability to recognize the protein independently of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving the two labeled antibodies and which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the protein’s phosphorylation state under a no-wash assay format.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003ePhospho-ALK (Tyr1604) two-plate assay protocol\u003cbr\u003e\nThe two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the additon of the Phospho-ALK Tyr1604) HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003ePhospho-ALK (Tyr1604) one-plate assay protocol\u003cbr\u003e\nDetection of  Phospho-ALK (Tyr1604) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required.\u003cbr\u003e\nThis HTS-designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nLysis Buffer Compatibility-Lysis Buffer 2 Lysis Buffer 4\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775250813097,"sku":"64ALKY04PEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-64alky04peg","provider":"Iright","version":"1.0","type":"link"}